The LH (Luteinizing Hormone) ELISA Kit is meticulously developed for the quantitative analysis of Luteinizing Hormone levels in various human samples. Luteinizing Hormone, a critical hormone involved in reproductive function, specifically regulating ovulation and estrogen production in females and testosterone production in males. Accurate measurement of LH is essential for assessing fertility, diagnosing reproductive disorders, and monitoring hormonal imbalances. This ELISA kit ensures exceptional sensitivity and specificity, providing reliable and reproducible results. Manufactured under stringent quality control measures, this kit delivers robust performance and is user-friendly, making it ideal for both research and clinical applications. Rely on Assay Genie's LH ELISA Kit for accurate and dependable quantification of this vital hormone in your studies.
Product Name:
Human LH (Luteinizing Hormone) ELISA Kit
SKU:
AEES00055
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 mIU/mL
Detection range:
0.16-10 mIU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
