Human Low Density Lipoprotein Receptor Related Protein 2 (LRP2) ELISA Kit
The Human Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) ELISA Kit is specifically designed for the accurate and precise measurement of LRP2 levels in human biological samples, including serum, plasma, and cell culture supernatants. With a high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for various research applications.LRP2, also known as megalin, is a cell surface receptor involved in the endocytosis and transport of various ligands, including lipoproteins and vitamins.
Dysregulation of LRP2 has been linked to several diseases, such as chronic kidney disease, neurodegenerative disorders, and metabolic syndromes, making it a crucial biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human LRP2 ELISA Kit offers researchers a valuable tool for investigating the role of LRP2 in health and disease, providing insights into its function and potential clinical implications.
Product Name:
Human Low Density Lipoprotein Receptor Related Protein 2 (LRP2) ELISA Kit
Product Code:
HUDL01754
Size:
96 Assays
Target:
LRP2
Synonyms:
DBS, gp330, Glycoprotein 330, Megalin, Low Density Lipoprotein-Related Protein 2
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
