The Human LTF (Lactoferrin) ELISA Kit is specifically designed for the precise measurement of lactoferrin levels in human samples such as serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for various research applications.Lactoferrin is a multifunctional protein involved in various biological processes, including immune response modulation, antimicrobial activity, and iron metabolism regulation. Its levels have been linked to a range of health conditions, including infections, inflammation, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human LTF (Lactoferrin) ELISA Kit is a reliable tool for researchers looking to explore the role of lactoferrin in human health and disease, providing a valuable insight into its potential as a diagnostic and therapeutic target.
Product Name:
Human LTF (Lactoferrin) ELISA Kit
SKU:
HUES03395
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
