Description
Human LYPLA1 Recombinant Protein (N-His)
Human LYPLA1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Asp230, with an N-terminal His tag. Predicted molecular weight: 26.83 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Acyl-protein thioesterase 1 (LYPLA1) is a ~24 kDa protein. Acts as an acyl-protein thioesterase. For research use only.
Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC).
| Product Name: | Human LYPLA1 Recombinant Protein (N-His) |
| SKU: | GXRP04754 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | APT1, LYPLA1, LPL-I, Lysophospholipase I, LysoPLA I, LPL1, hAPT1, Acyl-protein thioesterase 1, APT-1, Lysophospholipase 1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Asp230 |
| Calculated MW: | 26.83 kDa |
| UniProt: | O75608 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
