Description
Human MAD2L1 Recombinant Protein (N-His)
Human MAD2L1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Asp205, with an N-terminal His tag. Predicted molecular weight: 25.67 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) is a ~23 kDa protein. Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. For research use only.
In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
| Product Name: | Human MAD2L1 Recombinant Protein (N-His) |
| SKU: | GXRP04183 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | MAD2L1, Mitotic arrest deficient 2-like protein 1, HsMAD2, MAD2-like protein 1, Mitotic spindle assembly checkpoint protein MAD2A, MAD2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Asp205 |
| Calculated MW: | 25.67 kDa |
| UniProt: | Q13257 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
