Human Maltase Glucoamylase,Intestinal (MGA) ELISA Kit
The Human Maltase-Glucoamylase Intestinal (MGA) ELISA Kit is designed for the precise measurement of MGA levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an excellent tool for various research applications.Maltase-Glucoamylase Intestinal (MGA) is a key enzyme responsible for breaking down complex carbohydrates into simpler sugars in the intestine. It plays a vital role in digestion and nutrient absorption, making it crucial for overall gastrointestinal health.
Abnormal levels of MGA have been linked to digestive disorders and malabsorption issues, highlighting its importance as a potential biomarker for studying and treating these conditions.The Human Maltase-Glucoamylase Intestinal (MGA) ELISA Kit provides researchers with a reliable and efficient method for analyzing MGA levels, allowing for a better understanding of its function and potential implications in various gastrointestinal diseases. Get your kit today and advance your research in the field of digestive health.
Product Name:
Human Maltase Glucoamylase,Intestinal (MGA) ELISA Kit
Product Code:
HUDL01840
Size:
96 Assays
Target:
MGA
Synonyms:
MGAM, Glucan 1,4-Alpha-Glucosidase
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
