Description
Human MAP2K1 Recombinant Protein (N-His)
Human MAP2K1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu54-Val369, with an N-terminal His tag. Predicted molecular weight: 37.43 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) is a ~43 kDa protein. Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. For research use only.
Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.
| Product Name: | Human MAP2K1 Recombinant Protein (N-His) |
| SKU: | GXRP02032 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ERK activator kinase 1, MKK1, MAPK/ERK kinase 1, MAPKK 1, MAP kinase kinase 1, MEK 1, MEK1, Dual specificity mitogen-activated protein kinase kinase 1, PRKMK1, MAP2K1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu54-Val369 |
| Calculated MW: | 37.43 kDa |
| UniProt: | Q02750 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
