Description
Human MAPKAPK3 Recombinant Protein (N-His)
Human MAPKAPK3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Arg129-Asp329, with an N-terminal His tag. Predicted molecular weight: 25.48 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. MAP kinase-activated protein kinase 3 (MAPKAPK3) is a ~42 kDa protein. Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. For research use only.
Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36.
| Product Name: | Human MAPKAPK3 Recombinant Protein (N-His) |
| SKU: | GXRP02373 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | 3pK, MAPKAPK-3, MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3, MK-3, MAPKAPK3, Chromosome 3p kinase, MAPKAP-K3 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Arg129-Asp329 |
| Calculated MW: | 25.48 kDa |
| UniProt: | Q16644 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
