Description
Human MARCKS Recombinant Protein (N-His)
Human MARCKS Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Glu332, with an N-terminal His tag. Predicted molecular weight: 33.72 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Myristoylated alanine-rich C-kinase substrate (MARCKS) is a ~31 kDa protein. Membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis through lipid sequestering and/or protein docking to membranes. For research use only.
Thus, exerts an influence on a plethora of physiological processes, such as embryonic development, tissue regeneration, neuronal plasticity, and inflammation. Sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis. During inflammation, promotes the migration and adhesion of inflammatory cells and the secretion of cytokines such as tumor necrosis factor (TNF), particularly in macrophages. Plays an essential role in bacteria-induced intracellular reactive oxygen species (ROS) formation in the monocytic cell type.
| Product Name: | Human MARCKS Recombinant Protein (N-His) |
| SKU: | GXRP01385 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Myristoylated alanine-rich C-kinase substrate, 80K-L protein, PKCSL, Protein kinase C substrate, 80 kDa protein, light chain, PRKCSL, MACS, MARCKS |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Glu332 |
| Calculated MW: | 33.72 kDa |
| UniProt: | P29966 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
