Human Matrix Metalloproteinase 10 (MMP10) ELISA Kit
The Human Matrix Metalloproteinase 10 (MMP10) ELISA Kit from AssayGenie is a highly reliable and sensitive tool for quantifying MMP10 levels in human samples such as serum, plasma, and cell culture supernatants. With its innovative design, this kit provides accurate and reproducible results, making it suitable for a wide range of research applications.Matrix Metalloproteinase 10 (MMP10) is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a crucial role in tissue remodeling and various pathological processes including cancer metastasis and inflammatory diseases.
By measuring MMP10 levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.Don't miss out on the opportunity to study the role of MMP10 in human health and disease with the AssayGenie Human MMP10 ELISA Kit. Order yours today and take your research to the next level.
Product Name:
Human Matrix Metalloproteinase 10 (MMP10) ELISA Kit
Product Code:
HUDL01869
Size:
96 Assays
Target:
MMP10
Synonyms:
STMY2, SL2, Stromelysin 2, Transin-2
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
