Human Matrix Metalloproteinase 25 (MMP25) ELISA Kit
The Human Matrix Metalloproteinase-25 (MMP25) ELISA Kit is a cutting-edge tool for the precise measurement of MMP25 levels in various biological samples, including human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring the delivery of consistent and accurate results, thus making it an indispensable asset for a diverse range of scientific investigations.MMP25 is a key enzyme involved in extracellular matrix degradation, cell migration, and tissue remodeling processes. Its dysregulation has been linked to various pathological conditions, such as cancer, inflammatory diseases, and tissue fibrosis.
Therefore, the MMP25 ELISA Kit serves as a valuable resource for researchers studying these disorders and exploring potential therapeutic interventions.Overall, the MMP25 ELISA Kit offers a comprehensive solution for the precise quantification of MMP25 levels, enabling researchers to gain deeper insights into the intricate mechanisms underlying numerous physiological and pathological processes. With its superior performance and reliability, this kit is an essential tool for advancing scientific knowledge and driving innovation in the field of biomedical research.
Product Name:
Human Matrix Metalloproteinase 25 (MMP25) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
