Human Matrix Metalloproteinase 26 (MMP26) ELISA Kit
The Human Matrix Metalloproteinase-26 (MMP26) ELISA Kit is a powerful tool for the accurate measurement of MMP26 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Matrix metalloproteinase-26 (MMP26) is an important enzyme involved in tissue remodeling and extracellular matrix degradation. It plays a key role in processes such as wound healing, tissue repair, and cancer progression.
Elevated levels of MMP26 have been associated with various pathological conditions, making it a valuable biomarker for studying disease mechanisms and developing potential therapeutic interventions.Overall, the Human MMP26 ELISA Kit offers researchers a reliable and efficient solution for studying the role of MMP26 in health and disease. Its high performance and ease of use make it an essential tool for gaining valuable insights into the functions of this critical enzyme.
Product Name:
Human Matrix Metalloproteinase 26 (MMP26) ELISA Kit
Product Code:
HUDL01881
Size:
96 Assays
Target:
MMP26
Synonyms:
Matrilysin-2, Endometase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
