Description
Human MBD1 Recombinant Protein (N-His)
Human MBD1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Leu216, with an N-terminal His tag. Predicted molecular weight: 26.11 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Methyl-CpG-binding domain protein 1 (MBD1) is a ~66 kDa protein. Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. For research use only.
Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
| Product Name: | Human MBD1 Recombinant Protein (N-His) |
| SKU: | GXRP03040 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CXXC3, PCM1, CXXC-type zinc finger protein 3, Protein containing methyl-CpG-binding domain 1, Methyl-CpG-binding domain protein 1, MBD1, Methyl-CpG-binding protein MBD1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Leu216 |
| Calculated MW: | 26.11 kDa |
| UniProt: | Q9UIS9 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
