Description
Human MCM8 Recombinant Protein (N-His)
Human MCM8 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser90-Lys366, with an N-terminal His tag. Predicted molecular weight: 33.26 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA helicase MCM8 is a ~93 kDa protein. Component of the MCM8-MCM9 complex, which is involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). For research use only.
The MCM8-MCM9 complex is a 3'-5' DNA helicase and single-stranded (ss)DNA-stimulated ATPase which binds ssDNA in the presence of nucleoside triphosphates. Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs, probably by regulating the localization of the MNR complex. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression.
| Product Name: | Human MCM8 Recombinant Protein (N-His) |
| SKU: | GXRP04757 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | MCM8, C20orf154, Minichromosome maintenance 8, DNA helicase MCM8 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser90-Lys366 |
| Calculated MW: | 33.26 kDa |
| UniProt: | Q9UJA3 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
