Description
Human MECR Recombinant Protein (N-His)
Human MECR Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Arg42-Met373, with an N-terminal His tag. Predicted molecular weight: 38.36 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Enoyl-[acyl-carrier-protein] reductase, mitochondrial (MECR) is a ~40 kDa protein. Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). For research use only.
Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates. May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells. Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism.
| Product Name: | Human MECR Recombinant Protein (N-His) |
| SKU: | GXRP04758 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | MECR, NRBF-1, Nuclear receptor-binding factor 1, NBRF1, 2-enoyl thioester reductase, HsNrbf-1, Enoyl-[acyl-carrier-protein] reductase, mitochondrial |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Arg42-Met373 |
| Calculated MW: | 38.36 kDa |
| UniProt: | Q9BV79 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
