SynonymsPMEL,GP100,SI,SIL,SILV,Pmel17,Silver Homolog,Premelanosome Protein,Silver Locus Protein Homolog,95 kDa melanocyte-specific secreted glycoprotein
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL01825
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P40967
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PMEL,GP100,SI,SIL,SILV,Pmel17,Silver Homolog,Premelanosome Protein,Silver Locus Protein Homolog,95 kDa melanocyte-specific secreted glycoprotein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human Melanoma-Associated ME20 (ME20M) ELISA Kit is a powerful tool for detecting and quantifying levels of ME20M in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in various research applications.ME20M is a protein associated with melanoma, a type of skin cancer that arises from melanocytes. Elevated levels of ME20M have been observed in melanoma patients, making it a valuable biomarker for monitoring disease progression and response to treatment.
Understanding the role of ME20M in melanoma can help researchers develop more effective diagnostic and therapeutic strategies for this deadly disease.With its reliable performance and ease of use, the Human Melanoma-Associated ME20 (ME20M) ELISA Kit is an essential tool for researchers studying melanoma and seeking to improve patient outcomes.
Product Name:
Human Melanoma Associated ME20 (ME20M) ELISA Kit
Product Code:
HUDL01825
Size:
96 Assays
Target:
ME20M
Synonyms:
PMEL, GP100, SI, SIL, SILV, Pmel17, Silver Homolog, Premelanosome Protein, Silver Locus Protein Homolog, 95 kDa melanocyte-specific secreted glycoprotein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
