Description
Human METTL1 Recombinant Protein (N-His)
Human METTL1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr34-His276, with an N-terminal His tag. Predicted molecular weight: 30.06 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. tRNA (guanine-N(7)-)-methyltransferase (METTL1) is a ~31 kDa protein. Catalytic component of METTL1-WDR4 methyltransferase complex that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). For research use only.
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs. Internal N(7)-methylguanine methylation of mRNAs in response to stress promotes their relocalization to stress granules, thereby suppressing their translation.
| Product Name: | Human METTL1 Recombinant Protein (N-His) |
| SKU: | GXRP04428 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | miRNA (guanine-N(7)-)-methyltransferase, Methyltransferase-like protein 1, tRNA (guanine-N(7)-)-methyltransferase, mRNA (guanine-N(7)-)-methyltransferase, C12orf1, tRNA (guanine(46)-N(7))-methyltransferase, METTL1, tRNA(m7G46)-methyltransferase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr34-His276 |
| Calculated MW: | 30.06 kDa |
| UniProt: | Q9UBP6 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
