Description
Human MGMT Recombinant Protein (N-His)
Human MGMT Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Asn207, with an N-terminal His tag. Predicted molecular weight: 23.81 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Methylated-DNA--protein-cysteine methyltransferase (MGMT) is a ~21 kDa protein. Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. For research use only.
Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
| Product Name: | Human MGMT Recombinant Protein (N-His) |
| SKU: | GXRP01044 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | MGMT, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Asn207 |
| Calculated MW: | 23.81 kDa |
| UniProt: | P16455 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
