Human Microphthalmia Associated Transcription Factor (MITF) ELISA Kit
The Human Microphthalmia-associated Transcription Factor (MITF) ELISA Kit is specifically designed for the precise measurement of MITF levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable tool for a diverse range of research applications.MITF is a key transcription factor known for its role in regulating melanocyte development and function, as well as in controlling the expression of genes involved in pigment production.
Dysregulation of MITF has been linked to various diseases, including melanoma and other types of cancer, making it a valuable biomarker for studying disease progression and potential therapeutic interventions. By utilizing the MITF ELISA Kit, researchers can gain valuable insights into the role of this transcription factor in health and disease, ultimately advancing our understanding of melanocyte biology and the development of novel treatment strategies. Trust in the accuracy and reliability of the MITF ELISA Kit for your research needs.
Product Name:
Human Microphthalmia Associated Transcription Factor (MITF) ELISA Kit
Product Code:
HUDL01862
Size:
96 Assays
Target:
MITF
Synonyms:
WS2A, MI, bHLHe32, Homolog Of Mouse Microphthalmia, Class E basic helix-loop-helix protein 32
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
