SynonymsCSBP1,CSBP2,CSPB1,EXIP,Mxi2,PRKM14,PRKM15,RK,SAPK2A,P38,Mitogen-activated protein kinase p38 alpha,Cytokine suppressive anti-inflammatory drug-binding protein
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL01793
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q16539
Sensitivity:
0.56ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
CSBP1,CSBP2,CSPB1,EXIP,Mxi2,PRKM14,PRKM15,RK,SAPK2A,P38,Mitogen-activated protein kinase p38 alpha,Cytokine suppressive anti-inflammatory drug-binding protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Mitogen Activated Protein Kinase 14 (MAPK14) ELISA Kit
The Human Mitogen-Activated Protein Kinase 14 (MAPK14) ELISA Kit is a highly accurate and reliable tool for the quantitative detection of MAPK14 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reproducible results, making it suitable for various research applications.MAPK14, also known as p38 MAPK, plays a crucial role in various cellular processes, including inflammation, cell proliferation, and stress response.
Dysregulation of MAPK14 has been implicated in several diseases, including cancer, inflammatory disorders, and neurodegenerative diseases, making it a valuable biomarker for studying and potentially targeting these conditions.By utilizing the Human MAPK14 ELISA Kit, researchers can accurately measure MAPK14 levels in human samples, providing valuable insights into the role of this protein in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human Mitogen Activated Protein Kinase 14 (MAPK14) ELISA Kit
Product Code:
HUDL01793
Size:
96 Assays
Target:
MAPK14
Synonyms:
CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, P38, Mitogen-activated protein kinase p38 alpha, Cytokine suppressive anti-inflammatory drug-binding protein
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
