Description
Human MRE11 Recombinant Protein (C-His)
Human MRE11 Recombinant Protein (C-His) is a recombinant protein expressed in insect cells, spanning Met1-Arg708, with a C-terminal His tag. Predicted molecular weight: 81.56 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Double-strand break repair protein MRE11 is a ~80 kDa protein. Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. For research use only.
The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with RBBP8/CtIP to initiate end resection. 1. de Jager, M. et al. (2001) Molecular cell 8, 1129-35. PMID: 11741547 2. Carson, CT. et al. (2003) The EMBO journal 22, 6610-20. PMID: 14657032 3. Park, YB. et al. (2011) Structure (London, England : 1993) 19, 1591-602. PMID: 22078559 4. Limbo, O. et al. (2012) Nucleic acids research 40, 11435-49. PMID: 23080121 5. Shibata, A. et al. (2014) Molecular cell 53, 7-18. PMID: 24316220 6. Kijas, AW. et al. (2015) Nucleic acids research 43, 8352-67. PMID: 26240375 7. Anand, R. et al. (2016) Molecular cell 64, 940-950. PMID: 27889449 8. Myler, LR. et al. (2017) Molecular cell 67, 891-898.e4. PMID: 28867292 9. Coquel, F. et al. (2018) Nature 557, 57-61. PMID: 29670289 10. He, YJ. et al. (2018) Nature 563, 522-526. PMID: 30464262
| Product Name: | Human MRE11 Recombinant Protein (C-His) |
| SKU: | GXRP06801 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | HNGS1, Double-strand break repair protein MRE11, MRE11A, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog A, MRE11 homolog A, MRE11 homolog 1, MRE11, Meiotic recombination 11 homolog 1 |
| Tag: | C-His |
| Expression Host: | Insect cells |
| Protein Length: | Met1-Arg708 |
| Calculated MW: | 81.56 kDa |
| UniProt: | P49959 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
