SynonymsTBM,MUC5,LeB,Oligomeric Mucus/Gel-Forming,Mucin 5,Subtypes A And C,Tracheobronchial/Gastric,Gastric mucin,Lewis B blood group antigen,Major airway glycoprotein
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
HUDL01936
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P98088
Sensitivity:
28pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
TBM,MUC5,LeB,Oligomeric Mucus/Gel-Forming,Mucin 5,Subtypes A And C,Tracheobronchial/Gastric,Gastric mucin,Lewis B blood group antigen,Major airway glycoprotein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Human MUC5AC ELISA Kit is specifically designed for the precise measurement of MUC5AC levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.MUC5AC is a subtype of mucin that plays a critical role in protecting and lubricating the mucosal surfaces in the respiratory tract, gastrointestinal tract, and other mucin-producing tissues.
Abnormal levels of MUC5AC have been linked to various respiratory diseases, such as chronic obstructive pulmonary disease (COPD), asthma, and cystic fibrosis, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its robust performance and ease of use, the Human MUC5AC ELISA Kit is an indispensable tool for researchers and clinicians looking to explore the role of MUC5AC in mucosal immunity and disease pathogenesis.
Product Name:
Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit
Product Code:
HUDL01936
Size:
96 Assays
Target:
MUC5AC
Synonyms:
TBM, MUC5, LeB, Oligomeric Mucus/Gel-Forming, Mucin 5,Subtypes A And C,Tracheobronchial/Gastric, Gastric mucin, Lewis B blood group antigen, Major airway glycoprotein
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
