The Human MUC5AC (Mucin 5 subtype AC) ELISA Kit is specifically designed for the accurate quantification of MUC5AC levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MUC5AC is a key mucin protein responsible for maintaining the protective barrier of epithelial cells in mucosal tissues. Dysregulation of MUC5AC expression has been linked to various respiratory diseases, including asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis. Therefore, accurate detection and measurement of MUC5AC levels are crucial in understanding the pathogenesis of these diseases and developing potential therapeutic interventions.Overall, the Human MUC5AC ELISA Kit provides researchers with a valuable tool for studying the role of mucins in respiratory diseases and advancing the field of mucin biology research.
Product Name:
Human MUC5AC (Mucin 5 Subtype AC) ELISA Kit
SKU:
HUES03162
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
