Human MYBPC3 (Myosin Binding Protein C, Cardiac) CLIA Kit
The Human MYBPC3 (Myosin Binding Protein C, Cardiac) CLIA Kit is a cutting-edge assay designed for the accurate detection of MYBPC3 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.MYBPC3 is a critical protein involved in cardiac muscle function, specifically in regulating the contraction of heart muscle cells. Mutations in the MYBPC3 gene are associated with various cardiac conditions, including hypertrophic cardiomyopathy.
As such, accurate measurement of MYBPC3 levels can provide valuable insights into the pathophysiology of cardiac diseases and aid in the development of targeted therapies.With its advanced technology and precision, the Human MYBPC3 CLIA Kit is an indispensable tool for researchers studying cardiac biology and seeking to unravel the mechanisms underlying cardiac disorders. Trust in this kit to deliver accurate and dependable results for your research needs.
Product Name:
Human MYBPC3 (Myosin Binding Protein C, Cardiac) CLIA Kit
SKU:
HUES00455
Target:
Human MYBPC3 (Myosin Binding Protein C, Cardiac)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYBPC3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYBPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYBPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MYBPC3. You can calculate the concentration of Human MYBPC3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
102-121
103-115
Average (%)
92
110
109
1:4
Range (%)
103-118
84-98
98-113
Average (%)
109
91
105
1:8
Range (%)
92-105
94-108
98-117
Average (%)
97
100
106
1:16
Range (%)
93-106
99-112
102-119
Average (%)
99
104
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
97-111
103
Cell culture media (n=5)
95-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.54
184.96
687.68
99.78
180.8
704.4
Standard deviation
10.2
15.48
72.21
9.47
16.25
60.44
C V (%)
9.76
8.37
10.5
9.49
8.99
8.58
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MYBPC3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYBPC3 in samples. No significant cross-reactivity or interference between Human MYBPC3 and analogues was observed.
