Human Myelin Proteolipid Protein Like Protein (PLPL) ELISA Kit
The Human Myelin Proteolipid Protein-Like Protein (PLPL) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of PLPL levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an invaluable tool for researchers investigating the role of PLPL in various biological processes.PLPL is a key protein that shares similarities with myelin proteolipid protein, playing a critical role in the formation and maintenance of myelin, the protective sheath surrounding nerve fibers.
Dysregulation of PLPL has been linked to neurological disorders such as multiple sclerosis, making it a promising biomarker for studying and potentially treating these conditions.Whether you are exploring the role of PLPL in neurodegenerative diseases, investigating its potential as a therapeutic target, or simply looking to better understand its function in the body, the Human PLPL ELISA Kit offers a reliable and efficient solution for your research needs.
Product Name:
Human Myelin Proteolipid Protein Like Protein (PLPL) ELISA Kit
Product Code:
HUDL02347
Size:
96 Assays
Target:
PLPL
Synonyms:
C14orf162, CCDC177, Coiled-coil domain-containing protein 177
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
