Description
Human NANOG Recombinant Protein (N-His)
Human NANOG Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Trp153-Val305, with an N-terminal His tag. Predicted molecular weight: 19.82 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Homeobox protein NANOG is a ~34 kDa protein. Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. For research use only.
Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'.
| Product Name: | Human NANOG Recombinant Protein (N-His) |
| SKU: | GXRP02873 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Homeobox protein NANOG, NANOG, Homeobox transcription factor Nanog, hNanog |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Trp153-Val305 |
| Calculated MW: | 19.82 kDa |
| UniProt: | Q9H9S0 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
