Human Natriuretic Peptide Receptor 2 (NPR2) ELISA Kit
The Human Natriuretic Peptide Receptor 2 (NPR2) ELISA Kit is a reliable and accurate tool for the measurement of NPR2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and consistent results, making it an ideal choice for various research applications.NPR2 is a critical receptor involved in natriuretic peptide signaling, regulating blood pressure and fluid balance in the body. Dysregulation of NPR2 has been implicated in various cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
By using the Human NPR2 ELISA Kit, researchers can gain valuable insights into the role of NPR2 in cardiovascular health and disease, paving the way for new discoveries and advancements in the field of cardiovascular research. Trust in the accuracy and reliability of this kit to support your research endeavors and drive scientific progress.
Product Name:
Human Natriuretic Peptide Receptor 2 (NPR2) ELISA Kit
Product Code:
HUDL02072
Size:
96 Assays
Target:
NPR2
Synonyms:
AMDM, ANPRB, ANPb, NPRBi, NPR-B, GUCY2B, GUC2B, Guanylate Cyclase B, Atrial natriuretic peptide receptor type B
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
