Human NCAM/CD56 (Neural Cell Adhesion Molecule) CLIA Kit
The Human NCAM (CD56) CLIA Kit is specifically designed for the precise quantification of Neural Cell Adhesion Molecule (NCAM or CD56) levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and dependable results for a variety of research purposes.NCAM, also known as CD56, is a cell adhesion molecule that plays a crucial role in neural development and regeneration. It is involved in neuronal migration, synaptic plasticity, and cell signaling, making it a key player in the central nervous system.
Aberrant expression of NCAM has been linked to neurological disorders such as Alzheimer's disease, schizophrenia, and autism, highlighting its importance as a biomarker for diagnostic and therapeutic research.With its reliable performance and versatility, the Human NCAM (CD56) CLIA Kit is an indispensable tool for studying the role of NCAM in neurological diseases, neural development, and regenerative medicine. Discover the potential of NCAM in advancing research and developing innovative therapies with this advanced assay kit.
Product Name:
Human NCAM/CD56 (Neural Cell Adhesion Molecule) CLIA Kit
SKU:
HUES01043
Target:
Human NCAM/CD56 (Neural Cell Adhesion Molecule)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NCAM/CD56. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NCAM/CD56 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NCAM/CD56, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NCAM/CD56. You can calculate the concentration of Human NCAM/CD56 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
101-115
100-112
Average (%)
100
108
107
1:4
Range (%)
89-102
90-104
85-101
Average (%)
95
95
92
1:8
Range (%)
101-115
100-113
93-105
Average (%)
107
106
100
1:16
Range (%)
100-115
93-106
94-110
Average (%)
107
99
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
1.97
8.3
1.01
2.14
8.6
Standard deviation
0.09
0.17
0.89
0.08
0.19
0.91
C V (%)
8.41
8.63
10.72
7.92
8.88
10.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NCAM/CD56 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NCAM/CD56 in samples. No significant cross-reactivity or interference between Human NCAM/CD56 and analogues was observed.
