Description
Human NEDD8 Recombinant Protein (N-His-SUMO)
Human NEDD8 Recombinant Protein (N-His-SUMO) is a recombinant protein expressed in E. coli, spanning Leu2-Gln81, with an N-terminal His-SUMO tag. Predicted molecular weight: 21.28 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ubiquitin-like protein NEDD8 is a ~9 kDa protein. Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. For research use only.
Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.
| Product Name: | Human NEDD8 Recombinant Protein (N-His-SUMO) |
| SKU: | GXRP10847 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | NEDD-8, NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8 |
| Tag: | N-His-SUMO |
| Expression Host: | E. coli |
| Protein Length: | Leu2-Gln81 |
| Calculated MW: | 21.28 kDa |
| UniProt: | Q15843 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
