Human Nei Endonuclease VIII Like Protein 3 (NEIL-3) ELISA Kit
The Human NEIL3 (Endonuclease VIII-like Protein 3) ELISA Kit is meticulously crafted for the precise measurement of NEIL3 levels in human serum, plasma, and cell culture supernatants. This cutting-edge kit boasts exceptional sensitivity and specificity, ensuring the delivery of dependable and consistent results, thus making it the perfect tool for a diverse array of research endeavors.NEIL3 is a pivotal protein that plays a crucial role in DNA repair mechanisms, specifically in the removal of oxidized DNA bases.
Its intricate functions are implicated in various physiological processes and diseases, such as cancer, neurodegeneration, and oxidative stress-related conditions. Therefore, the accurate assessment of NEIL3 levels is indispensable for elucidating these disease mechanisms and advancing potential therapeutic interventions.
Product Name:
Human Nei Endonuclease VIII Like Protein 3 (NEIL-3) ELISA Kit
Product Code:
HUDL02008
Size:
96 Assays
Target:
NEIL
Synonyms:
FPG2, hFPG2, hNEI3, Endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Nei-like protein 3
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
