Human Neuroblastoma,Suppression Of Tumorigenicity 1 (NBL1) ELISA Kit
The Human Neuroblastoma Suppression of Tumorigenicity 1 (NBL1) ELISA Kit is a valuable tool for researchers studying neuroblastoma and its associated conditions. This kit allows for the precise measurement of NBL1 levels in human serum, plasma, and cell culture supernatants, providing reliable and reproducible results.NBL1 is a key protein involved in suppressing tumorigenicity in neuroblastoma, making it a critical biomarker for understanding the mechanisms of this disease and potentially developing targeted therapies.
Its role in neurodegenerative disorders further highlights its importance in research and clinical applications.With its high sensitivity and specificity, the Human NBL1 ELISA Kit is an essential resource for researchers looking to advance their understanding of neuroblastoma and related conditions. Trust in this kit for accurate and insightful results that can drive innovation and progress in the field.
Product Name:
Human Neuroblastoma,Suppression Of Tumorigenicity 1 (NBL1) ELISA Kit
Product Code:
HUDL01991
Size:
96 Assays
Target:
NBL1
Synonyms:
NB, DAN, NO3, DAND1, Differential Screening-Selected Gene Aberrant In Neuroblastoma, DAN domain family member 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
