The Human Neuropeptide S Receptor (NPSR) ELISA Kit is a cutting-edge tool for the precise measurement of Neuropeptide S Receptor levels in human biological samples such as serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The Neuropeptide S Receptor is a vital component in the nervous system, playing a key role in regulating various physiological processes including stress response, anxiety, and pain perception.
Dysregulation of NPSR has been linked to psychiatric disorders such as anxiety and depression, highlighting its importance as a potential therapeutic target.With the Human Neuropeptide S Receptor (NPSR) ELISA Kit, researchers can gain valuable insights into the role of NPSR in health and disease, paving the way for innovative treatments and interventions in the field of neuroscience and mental health. Trust in the reliability and precision of this ELISA kit to drive your research forward.
Product Name:
Human Neuropeptide S Receptor (NPSR) ELISA Kit
Product Code:
HUDL02075
Size:
96 Assays
Target:
NPSR
Synonyms:
ASRT2, GPR154, GPRA, NPSR1, PGR14, VRR1, G Protein-Coupled Receptor For Asthma Susceptibility, G Protein-Coupled Receptor 154, G-protein coupled receptor PGR14
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
