Human Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit
The Human Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of NPY5R levels in human serum, plasma, and cell culture supernatants. This kit is ideal for a wide range of research applications, offering reliable and reproducible results.Neuropeptide Y Receptor Y5 (NPY5R) is a key player in the regulation of appetite, energy balance, and stress response.
Dysregulation of this receptor has been implicated in conditions such as obesity, eating disorders, and anxiety disorders. The NPY5R ELISA Kit provides researchers with a valuable tool for studying the role of this receptor in various physiological and pathological processes, potentially leading to the development of new therapeutic strategies.
Product Name:
Human Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit
Product Code:
HUDL02082
Size:
96 Assays
Target:
NPY5R
Synonyms:
NPYR5, NPY-Y5 receptor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
