The Human NGF (Nerve Growth Factor) ELISA Kit is specifically designed for the quantitative detection of NGF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for various research purposes.NGF is a key protein that plays a vital role in the growth and maintenance of nerve cells, making it a valuable biomarker for studying neurodevelopmental disorders, neurodegenerative diseases, and neurological conditions. By accurately measuring NGF levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially develop new therapeutic strategies.Overall, the Human NGF ELISA Kit is a valuable tool for researchers looking to investigate the role of NGF in various physiological and pathological processes, ultimately leading to a better understanding of nerve cell growth and function.
Product Name:
Human NGF (Nerve Growth Factor) ELISA Kit
SKU:
HUES02278
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
