Human Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit
The Human Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit is a specialized assay designed for the accurate quantification of NOSIP levels in human samples, including serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable tool for researchers in various fields.NOSIP is a critical protein that interacts with nitric oxide synthase, playing a key role in regulating nitric oxide production and signaling pathways.
Dysregulation of NOSIP has been implicated in various diseases, including cardiovascular disorders, neurological conditions, and inflammatory disorders. By measuring NOSIP levels, researchers can gain insights into the underlying mechanisms of these diseases and potentially develop new therapeutic interventions.Overall, the Human NOSIP ELISA Kit offers a convenient and efficient method for studying the role of NOSIP in health and disease, providing valuable information for advancing scientific research and clinical applications.
Product Name:
Human Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit
Product Code:
HUDL02059
Size:
96 Assays
Target:
NOSIP
Synonyms:
CGI-25, eNOS-interacting protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
