The Human NK6 Homeobox Protein 1 (NKX6-1) ELISA Kit is specifically designed for the accurate measurement of NKX6-1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.NKX6-1 is a critical transcription factor involved in the development and function of various organs, including the pancreas, central nervous system, and gastrointestinal tract.
Dysregulation of NKX6-1 has been linked to disorders such as diabetes, Parkinson's disease, and certain types of cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.By using the Human NK6 Homeobox Protein 1 (NKX6-1) ELISA Kit, researchers can accurately quantify NKX6-1 levels in biological samples, advancing our understanding of the role of this protein in health and disease.
Product Name:
Human NK6 Homeobox Protein 1 (NKX6-1) ELISA Kit
Product Code:
HUDL02046
Size:
96 Assays
Target:
NKX6-1
Synonyms:
NKX6A, Nkx6.1, NK6 Transcription Factor Related,Locus 1, Homeobox protein NK-6 homolog A
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
