Description
Human NME1 Recombinant Protein (N-His)
Human NME1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Glu152, with an N-terminal His tag. Predicted molecular weight: 19.31 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Nucleoside diphosphate kinase A (NME1) is a ~17 kDa protein. Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis. For research use only.
Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking it to isoprenoid metabolism. Additionally, functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups from its active site to target proteins. May function as a Mg(2+)-dependent single-stranded DNA endonuclease as part of the SET complex, cooperating with the 3'-5' exonuclease TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes. Reported to nick one DNA strand, enabling TREX1 to remove nucleotides from the free 3' end, enhancing DNA damage and suppressing DNA end reannealing and repair.
| Product Name: | Human NME1 Recombinant Protein (N-His) |
| SKU: | GXRP04434 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | NDPKA, NME1, NDP kinase A, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, NM23, Metastasis inhibition factor nm23, GAAD, Tumor metastatic process-associated protein, NDK A, NM23-H1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Glu152 |
| Calculated MW: | 19.31 kDa |
| UniProt: | P15531 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
