Human NOD2 (Nucleotide-binding oligomerization domain-containing protein 2) ELISA Kit
The Human NOD2 (Nucleotide-binding Oligomerization Domain containing protein 2) ELISA Kit is specifically designed for the accurate detection of NOD2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a range of research applications. NOD2 is a critical protein involved in the immune system, specifically in recognizing bacterial components and activating immune responses. Dysregulation of NOD2 has been associated with inflammatory diseases such as Crohn's disease and various autoimmune disorders.
Therefore, accurate measurement of NOD2 levels is key for understanding the underlying mechanisms of these diseases and potential therapeutic interventions. This ELISA kit is an essential tool for researchers studying immune responses, inflammation, and autoimmune diseases, providing valuable insights into the role of NOD2 in human health and disease. With its user-friendly protocol and reliable performance, the Human NOD2 ELISA Kit is a valuable addition to any research laboratory.
Product Name:
Human NOD2 (Nucleotide-binding oligomerization domain-containing protein 2) ELISA Kit
SKU:
HUFI07499
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
91-102%
84-96%
80-96%
EDTA Plasma (n = 5)
85-104%
88-98%
83-98%
Heparin Plasma (n = 5)
87-99%
87-98%
82-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-105
94
EDTA Plasma (n = 5)
89-103
94
Heparin Plasma (n = 5)
89-105
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
