Description
Human NONO Recombinant Protein (N-His)
Human NONO Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Arg73-Arg256, with an N-terminal His tag. Predicted molecular weight: 23.25 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Non-POU domain-containing octamer-binding protein (NONO) is a ~54 kDa protein. DNA- and RNA binding protein, involved in several nuclear processes. For research use only.
Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b.
| Product Name: | Human NONO Recombinant Protein (N-His) |
| SKU: | GXRP04235 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | 54 kDa nuclear RNA- and DNA-binding protein, NRB54, p54nrb, NMT55, NonO protein, p54(nrb), 55 kDa nuclear protein, NONO, DNA-binding p52/p100 complex, 52 kDa subunit, Non-POU domain-containing octamer-binding protein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Arg73-Arg256 |
| Calculated MW: | 23.25 kDa |
| UniProt: | Q15233 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
