Human NOV (Nephroblastoma overexpressed) ELISA Kit
The Human NOV (Nephroblastoma Overexpressed) ELISA Kit from AssayGenie is a cutting-edge tool for the precise quantification of NOV levels in human biological samples such as serum, plasma, and cell culture supernatants. This advanced kit is characterized by exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications in the field of nephroblastoma and related pathologies. NOV, also known as nephroblastoma overexpressed gene, is a key protein involved in various cellular processes, including cell growth, differentiation, and tumorigenesis.
It is particularly associated with nephroblastoma, a type of kidney cancer commonly found in children. Understanding the expression and function of NOV can provide valuable insights into the mechanisms underlying nephroblastoma development and progression, as well as potential therapeutic targets for this condition. With its state-of-the-art technology and reliable performance, the Human NOV ELISA Kit from AssayGenie is an invaluable asset for researchers aiming to unravel the complex biology of NOV and its implications in nephroblastoma and other related disorders.
Product Name:
Human NOV (Nephroblastoma overexpressed) ELISA Kit
SKU:
AEFI00093
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
82-97%
86-97%
85-101%
EDTA Plasma (n = 5)
81-100%
83-94%
80-94%
Heparin Plasma (n = 5)
85-104%
90-100%
89-103%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
90-105
90
EDTA Plasma (n = 5)
88-103
94
Heparin Plasma (n = 5)
88-100
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
