Description
Human NOVA1 Recombinant Protein (N-His)
Human NOVA1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala295-Gly507, with an N-terminal His tag. Predicted molecular weight: 23.10 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. RNA-binding protein Nova-1 (NOVA1) is a ~51 kDa protein. RNA-binding protein which regulates alternative splicing of pre-mRNAs in the brain and spinal cord in a sequence-specific manner. For research use only.
Binds to 5'-YCAY-3' repeats, with a minimum of 2 to 3 repeats necessary for high-affinity binding. Mediates both exon inclusion and exclusion, depending upon the position of its binding site within the pre-mRNA. Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly. Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 small nuclear ribonucleoprotein (snRNP) binding and inhibiting exon inclusion, whereas binding to an intronic 5'-YCAY-3' repeat enhances spliceosome assembly and favors exon inclusion.
| Product Name: | Human NOVA1 Recombinant Protein (N-His) |
| SKU: | GXRP01740 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Ventral neuron-specific protein 1, Onconeural ventral antigen 1, Neuro-oncological ventral antigen 1, RNA-binding protein Nova-1, Paraneoplastic Ri antigen, NOVA1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala295-Gly507 |
| Calculated MW: | 23.10 kDa |
| UniProt: | P51513 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
