The Human NOX3 (NADPH oxidase 3) ELISA Kit is a powerful tool designed for the precise measurement of NOX3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is renowned for its exceptional sensitivity and specificity, guaranteeing accurate and consistent results, thus proving to be indispensable for various research endeavors. NOX3, part of the NADPH oxidase family, plays a critical role in cellular redox signaling, particularly in generating reactive oxygen species (ROS) that regulate various physiological processes. Dysregulation of NOX3 has been implicated in numerous health conditions, including inflammation, cardiovascular diseases, and neurodegenerative disorders, positioning it as a valuable biomarker for investigating these diseases and developing potential therapeutic interventions.
Powered by advanced technology and meticulous design, the Human NOX3 ELISA Kit from Assay Genie offers researchers a reliable and efficient means to quantify NOX3 levels accurately, paving the way for deeper insights into the intricate mechanisms of redox biology and associated pathologies.
Product Name:
Human NOX3 (NADPH oxidase 3) ELISA Kit
SKU:
AEFI00087
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-103%
82-99%
82-101%
EDTA Plasma (n = 5)
82-94%
81-94%
81-95%
Heparin Plasma (n = 5)
83-99%
89-105%
88-105%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-104
95
EDTA Plasma (n = 5)
85-104
97
Heparin Plasma (n = 5)
85-102
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
