Description
Human NQO1 Recombinant Protein (N-His)
Human NQO1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Lys274, with an N-terminal His tag. Predicted molecular weight: 33.03 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. NAD(P)H dehydrogenase [quinone] 1 (NQO1) is a ~30 kDa protein. Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. For research use only.
In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species. Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion.
| Product Name: | Human NQO1 Recombinant Protein (N-His) |
| SKU: | GXRP01022 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | NAD(P)H:quinone oxidoreductase 1, Azoreductase, Phylloquinone reductase, NQO1, DT-diaphorase, NAD(P)H dehydrogenase [quinone] 1, QR1, DIA4, NMOR1, Quinone reductase 1, Menadione reductase, DTD |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Lys274 |
| Calculated MW: | 33.03 kDa |
| UniProt: | P15559 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
