The Human NRP1 (Neuropilin 1) ELISA Kit is expertly designed for the accurate quantification of Neuropilin 1 (NRP1) levels in various biological samples. NRP1 is an essential transmembrane receptor that plays versatile roles in various cellular functions, including angiogenesis, neuronal development, immune regulation, and cancer progression. As a multifunctional receptor involved in signaling pathways, NRP1 contributes significantly to physiological processes and pathologies, making it a key target for research in multiple fields. Our NRP1 ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results that meet the highest standards of accuracy. Manufactured under stringent quality controls, this kit guarantees robust performance and ease of use, making it the ideal choice for leading-edge research applications. Trust Assay Genie's NRP1 ELISA Kit for reliable and dependable quantification of this crucial receptor in your scientific inquiries.
Product Name:
Human NRP1 (Neuropilin 1) ELISA Kit
SKU:
AEES00167
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
