Description
Human NSUN2 Recombinant Protein (N-His)
Human NSUN2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning His415-Arg767, with an N-terminal His tag. Predicted molecular weight: 41.47 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. RNA cytosine C(5)-methyltransferase NSUN2 is a ~86 kDa protein. RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). For research use only.
Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C(5)-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C(5)-methylation of mitochondrial tRNAs.
| Product Name: | Human NSUN2 Recombinant Protein (N-His) |
| SKU: | GXRP03459 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Misu, NSUN2, hTrm4, RNA cytosine C(5)-methyltransferase NSUN2, TRM4, NOL1/NOP2/Sun domain family member 2, tRNA methyltransferase 4 homolog, mRNA cytosine C(5)-methyltransferase, SAKI, Substrate of AIM1/Aurora kinase B, Myc-induced SUN domain-containing protein, tRNA cytosine C(5)-methyltransferase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | His415-Arg767 |
| Calculated MW: | 41.47 kDa |
| UniProt: | Q08J23 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
