Human Nuclear Respiratory Factor 1 (NRF1) ELISA Kit
The Human Nuclear Respiratory Factor 1 (NRF1) ELISA Kit is specifically designed for the quantitative measurement of NRF1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an invaluable tool for researchers in various fields.NRF1 is a vital transcription factor that plays a crucial role in regulating the expression of nuclear genes involved in mitochondrial biogenesis and function. Dysregulation of NRF1 has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic disorders.
As such, monitoring NRF1 levels can offer insights into disease progression and potential therapeutic interventions.This NRF1 ELISA Kit provides a reliable and efficient method for measuring NRF1 levels, allowing researchers to gain a better understanding of its role in various biological processes and diseases. With its user-friendly protocol and high-performance characteristics, this kit is a valuable asset for studies aiming to investigate NRF1 biology and potential therapeutic targets.
Product Name:
Human Nuclear Respiratory Factor 1 (NRF1) ELISA Kit
Product Code:
HUDL02087
Size:
96 Assays
Target:
NRF1
Synonyms:
ALPHA-PAL, EWG, Alpha Palindromic-Binding Protein
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
