Human O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit
The Human O-6-Methylguanine-DNA Methyltransferase (MGMT) ELISA Kit is specially designed for the precise quantification of MGMT levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it suitable for a wide range of research applications.MGMT is a key enzyme involved in DNA repair, specifically in the removal of alkyl groups from the O-6 position of guanine. This critical function helps protect cells from DNA damage caused by alkylating agents.
Dysregulation of MGMT has been linked to several diseases, including cancer and neurological disorders, emphasizing its importance as a biomarker for studying these conditions and exploring potential therapeutic strategies.The Human O-6-Methylguanine-DNA Methyltransferase (MGMT) ELISA Kit is an invaluable tool for researchers aiming to study the role of MGMT in disease pathogenesis and treatment outcomes. Its high performance and reliability make it a preferred choice for laboratories conducting cutting-edge research in the field of DNA repair and molecular biology.
Product Name:
Human O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
