The Human OPN (Osteopontin) ELISA Kit is specifically designed for the precise measurement of osteopontin levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Osteopontin is a key protein involved in bone metabolism, immune system regulation, and inflammation. It plays a crucial role in various diseases such as cancer, cardiovascular diseases, and autoimmune disorders, making it a valuable biomarker for research and therapeutic development.With its high-quality components and user-friendly protocol, the Human OPN ELISA Kit from Assay Genie is a reliable tool for scientists and researchers studying osteopontin and its impact on health and disease.
Product Name:
Human OPN (Osteopontin) ELISA Kit
SKU:
HUES02393
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
