Description
Human OXCT1 Recombinant Protein (N-His)
Human OXCT1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr40-Leu489, with an N-terminal His tag. Predicted molecular weight: 50.99 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (OXCT1) is a ~56 kDa protein. Key enzyme for ketone body catabolism. For research use only.
Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production. Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
| Product Name: | Human OXCT1 Recombinant Protein (N-His) |
| SKU: | GXRP01844 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | 3-oxoacid CoA-transferase 1, OXCT1, Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, OXCT, SCOT, SCOT-s |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr40-Leu489 |
| Calculated MW: | 50.99 kDa |
| UniProt: | P55809 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
