The Human p38 MAPK ELISA Kit is specially designed for the precise measurement of p38 MAPK levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides highly accurate and consistent results, making it an ideal tool for a variety of research purposes. p38 MAPK, also known as mitogen-activated protein kinase, is a key signaling molecule involved in cellular responses to various stress stimuli. Its activation plays a critical role in processes such as inflammation, immune response, and cell proliferation.
Dysregulation of p38 MAPK signaling is implicated in numerous diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, underscoring the importance of studying its levels and activity. By using the Human p38 MAPK ELISA Kit, researchers can gain valuable insights into the role of p38 MAPK in health and disease, facilitating the development of novel therapeutic strategies. Trust in the reliability and precision of this ELISA kit to advance your research efforts in the field of p38 MAPK signaling.
Product Name:
Human p38 MAPK ELISA Kit
SKU:
HUFI02083
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-103%
87-100%
86-101%
EDTA Plasma (n = 5)
88-94%
82-97%
89-101%
Heparin Plasma (n = 5)
82-98%
84-98%
82-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
92-105
95
EDTA Plasma (n = 5)
88-102
94
Heparin Plasma (n = 5)
90-105
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
TMB Substrate
5 ml
10 ml
2-8°C (Avoid direct light)
Sample Dilution Buffer
10 ml
20 ml
2-8°C
Antibody Dilution Buffer
5 ml
10 ml
2-8°C
SABC Dilution Buffer
5 ml
10 ml
2-8°C
Stop Solution
5 ml
10 ml
2-8°C
Wash Buffer(25X)
15 ml
30 ml
2-8°C
Plate Sealer
3 pieces
5 pieces
-
Technical Manual
1 copy
1 copy
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Zahra, H, O. et al.
Circulating miRNA-122 and miRNA-223 expression signatures across TNM stages of hepatocellular carcinoma in Egyptian patients: association with angiogenesis, apoptosis, and autophagy-related biomarker
